ABI’s quantitated viral load controls from Source BioScience are intended for use in nucleic acid extraction and amplification processes alongside unknown samples to determine extraction efficiency, measure viral load of unknown samples, and validate, monitor, and troubleshoot molecular testing procedures.
Additionally, these new products are useful in training laboratory personnel, developing and validating new molecular testing methods, and in spiking studies.
The finished products contain purified, whole virus particles suspended in TNE with 0.1% sodium azide at a known concentration of 1.5 x 107virus particles (vp)/ml. Testing methods should include an extraction step to release viral DNA, followed by quantitation of the viral DNA. Viral DNA can be quantitated by real-time PCR using ABI’s Quantitated PCR Controls and Primers.
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