ABI- Advanced Biotechnologies FAQ


ABI- Advanced Biotechnologies

1. What is meant by DNA/RNA Copy Numbers in the non-quantitated PCR controls?
These "non-quantitated" PCR controls are available in 100ul volumes. They are not quantitated on the Roche LightCycler as the 250ul "Amplicheck quantitated Controls" are. However, one can calculate the copy number for these controls using a formula which uses the molecular weight of the specific DNA/RNA in question, the concentration (50ng/ul), and Avogadro's number. Attached you will find a table which gives the calculated results for several of these controls. Remember that these numbers are the result of an empirical calculation, they are not actually verified by testing.