Xenopus gene collection

The Xenopus Gene Collection (XGC) project is trans-NIH initiative, which was launched, as a subproject of the existing Mammalian Gene Collection. The general approach is to acquire or construct high-quality cDNA libraries. The libraries are screened by sequencing the 5' end of the clones. Putative full open reading frames clones are identified, and finally, selected clones are full-length sequence verified. The goal of the project is to make available a complete set of these full-length cDNA clones in Xenopus laevis and Xenopus tropicalis.


The XGC plan of work is:

  • The generation of libraries from different stages of development and adult tissues.

  • Sequencing of ~3,000 to 10,000 5' and 3' ESTs from each library.

  • All sequences are deposited into GenBank. The corresponding clones are available through the IMAGE clone distribution network and are fully accessible to the community.

  • Analysis of all available sequences into UniGene clusters.

  • Selection of candidate complete open reading frame (ORF) clones and their sequencing.

In summary a Xenopus Gene Collection (XGC) clone is identified by:

  • A unique IMAGE identifier [IMAGE ID] (cross-referring to one or more EST files in dbEST)

  • A unique MGC identifier [MGC_ID] (cross-referring to a sequence file in GenBank of the entire clone insert)


Description of rearrayed plates containing sequenced full-length MGC clones:


IRBH X. laevis Vectors confer ampicillin resistance
IRBG X. laevis Vectors confer chloramphenicol resistance
IRBN X. tropicalis Vectors confer ampicillin resistance


NB: In each of these subsets all clones on one plate share the same vector ( IRBH, IRBG and IRBN).

Details of the vectors used can be found here

Clones from the three subsets can be ordered as individual clones (streaks on agar) and 96-well plates (frozen bacterial stocks in glycerol).

Clones are ordered using the IMAGE ID on the standard I.M.A.G.E. order form (you cannot use the XGC_ID). If the clone ordered forms part of a XGC subset in the Source BioScience LifeSciences collection, you will receive the clone streaked out from IRBH, IRBG, or IRBN plates. Such clones will also have an XGC_ID which will be given on the email confirmation of your order. If a clone has an XGC_ID but is not yet part of the subset plates received by Source BioScience LifeSciences the clone will be streaked out from the main I.M.A.G.E. collection.