ORF Shuttle Clones


Full ORF (Open Reading Frame) shuttle clones contain the complete coding sequences for genes of interest - exactly in frame, from start to stopcodon. Due to the flexible Gateway® cloning system, the Shuttle Clones are a flexible source for protein expression in E. coli, yeast, the baculo system and mammalian cells. You can create your own expression clones by shuttling the ORF into the desired Gateway® expression vector.

All inserts are fully sequence verified, in particular: no frame shift, no stopcodon in frame.

To speed up your research, choose the ready-to-use Full ORF Expression Clone.

Full ORF Shuttle Clones 2

How to find full ORF clones

You can search by:

  • Keywords or general IDs (like accession number, clone ID,...) using GenomeCUBE
  • Sequence similarity (Blast)

We are continuously expanding our Full ORF Clone Collection. If a clone for your gene of interest is not available, please contact us


Full ORF Clones are the preferred source for in-depth gene analysis. Fields of application are:

  • Recombinant protein expression in various systems
  • Protein expression using fusion tags (N- and/or C-terminal)
  • Functional assays
  • Protein characterization
  • Protein localization studies
  • In vitro transcription
  • Easy shuttling of ORFs for advanced research

To express your gene of interest

  1. Choose the corresponding flexible Full ORF Shuttle Clone with Gateway® entry vector.
  2. Generate your own expression clone by shuttling the ORF into the Gateway® expression vector of your choice.
  3. Introduce your expression clone into the appropriate host and express the protein.

Many ORFs are available with or without stopcodon - annotated in your search hit. The availability of both formats provides the freedom of choice to produce native proteins or C-terminal fusion constructs.