The Cloning of Open Reading Frames
The arrival of systems biology requires the cloning of large sets of protein-encoding open reading frames (ORFs), to allow for the functional studies of the resultant proteomes. The CORF has generated and made available a set of clones covering the predicted ORF′s for human chromosome 22.
Ian Dunham′s team, at The Wellcome Trust Sanger Institute, Hinxton, UK has constructed the set of clones in a pENTR vector. The main advantage of using a recombinational cloning system is the ease with which each ORF can be transferred from the Entry Vector into any vector of choice by using the Gateway (Invitrogen) cloning method.
Source BioScience offers the complete clone set of 6 96-well microtitre plates, or individual clones, for research purposes only. The clones have been submitted to EMBL nucleotide sequence database.
We can also make subsets of your chosen clones from the collection, please contact us to discuss your requirements.
A genome annotation-driven approach to cloning the human ORFeome. Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I. Genome Biol. 2004;5(10):R84. Epub 2004 Sep 30.