Source BioScience are happy to announce the distribution a second, fully indexed mouse resource, constructed in Alan Bradley′s lab, The Wellcome Trust Sanger Institute, Hinxton, UK.
The library comprises of more than 93 thousand, constructed, insertional targeting vectors from two different libraries MHPP & MHPN. Approximately 6 thousand of these vectors can be used directly to inactivate genes, with an average targeting efficiency of 28%.
By using the combination of MHPP & MHPN vectors, 3′ Hprt and 5′ Hprt respectively, the disruption of both alleles of a gene may be analysed. Furthermore, larger genomic changes may be engineered, such as deletions, duplications translocations or inversions.
Each clone is a ready made targeting vector, incorporating a drug resistance gene for drug targeting, the 5′ or 3′ portion of the Hprt mini-gene, a loxP site for chromosome engineering. Finally, a mouse coat-colour marker, for stock maintenance is a present on each clone.
MICER clones are displayed on the Ensembl genome browser within a DAS (Distributed Annotation Server). End sequenced vectors are mapped to the C57BL/6J mouse genome assembly. 3′Hprt [MHPP] clones are displayed as blue or green, whilst 5′Hprt [MHPN] clones are displayed as black or red.
The MICER indexed library is a valuable tool that can facilitate the rapid disruption of many genes, therefore providing a powerful tool for accelerating genomic analysis.
If you would like further information regarding this resource, please go to the following URL: www.sanger.ac.uk/PostGenomics/mousegenomics/
Source BioScience offers the complete clone set of 821 384-well microtitre plates, or individual clones, for research purposes only.
We can also array subsets of your chosen clones from the collection. Please contact us to discuss your requirements.
Please note, the backbone vectors p5′ Hprt [MHPN] and p3′ Hprt [MHPP] are also available at Source BioScience LifeSciences.
Mutagenic insertion and chromosome engineering resource (MICER). Adams DJ, Biggs PJ, Cox T, Davies R, van der Weyden L, Jonkers J, Smith J, Plumb B, Taylor R, Nishijima I, Yu Y, Rogers J, Bradley A. Nat Genet. 2004 Aug;36(8):867-71. Epub 2004 Jul 4. The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambs, CB10 1SA, UK.