CF is a disorder of the cells that line the lungs, small intestines, sweat glands and pancreas. Sticky, thick mucus contributes to the destruction of lung tissue and impedes gas exchange in the lungs. It also prevents nutrient absorption in the small intestines, and blocks pancreatic ducts from releasing digestive enzymes. Approximately 85% of all people with CF cannot properly digest their food without supplemental enzymes.
A collection of ~52,000 human CF clones, produced by Bento Soares, is now available from Source BioScience LifeSciences providing a valuable resource for Cystic Fibrosis research. The clones have been constructed using a variety of tissues, viz., primary lung epithelial cells, normal lung tissue, primary lung cystic fibrosis epithelial cells and lung epithelial cells.
All clones have been cloned directionally into pT7T3-Pac vector and propagated in E. coli DH10B cells (T1 phage resistant).
This resource is available as bacterial glycerol stocks and can be ordered as individual clones, individual 384-well plates or complete set of 163 384-well plates.